Reversible Nematostatic Effect of Peganum harmala L. (Nitrariaceae) on Meloidogyne javanica

Effect of Syrian Rue on Root-Knot Nematodes

Authors

  • EL HASSAN MAYAD
  • KHADIJA BASAID
  • JAMES NICHOLAS FURZE
  • NIAMA HEIMEUR
  • BTISSAM SENHAJI
  • BOUCHRA CHEBLI
  • MILOUD EL HADEK
  • THIERRY MATEILLE
  • LALLA AMINA IDRISSI HASSANI
  • ZAHRA FERJI

Abstract

Meloidogyne javanica is considered as the most damaging nematode of vegetables in Morocco. Eco-friendly bionematicides are urgently required for its control. In vitro experiments were carried out to assess the direct effect of bioproducts of P. harmala against M. javanica. The bioassay showed extracts to be nematotoxic. Aqueous extracts of P. harmala exhibited reversible nematostatic activity. The estimated ID50 of the most active product in methanolic extracts was 368 ppm. HPLC-MS of the methanolic extract revealed that total content of major alkaloids of P. harmala was approximately 12.162±0.637mg/g. Harmine (8.514±0.521 mg/g) is the dominant alkaloid. In conclusion, P. harmala has a reversible nematostatic activity on second stage juveniles of M. javanica. The effect of P. harmala is due to its possession of a high content of β-carboline alkaloids, which warrant   further experimentation. Bioproducts from P. harmala should be exploited through formulations for management of the root knot nematode.


KEYWORDS: Peganum harmala,  Phytonematodes, in vitro, nematicide, alkaloids.

Author Biographies

EL HASSAN MAYAD

Laboratory of Biotechnology and Valorisation of Natural Resources, Department of Biology, Faculty of Sciences of Agadir, Ibn Zohr University,  BP 8106, 80000 Agadir, Morocco, Laboratory of Plant Biotechnology, Department of Biology, Faculty of Sciences of Agadir, Ibn Zohr University, BP 8106, 80000 Agadir, Morocco,  Department of Plant Protection, Nematology. Agronomic and Veterinary Institute Hassan II, Agadir, Morocco,Biotechnology and Environmental Engineering Team, Laboratory of Mechanic Process Energy and Environment, National School of Applied Sciences, Ibn Zohr University, PO Box: 1136/S, 80000, Agadir, Morocco

KHADIJA BASAID

Laboratory of Biotechnology and Valorisation of Natural Resources, Department of Biology, Faculty of Sciences of Agadir, Ibn Zohr University, BP 8106, 80000 Agadir, Morocco, Biotechnology and Environmental Engineering Team, Laboratory of Mechanic Process Energy and Environment, National School of Applied Sciences, Ibn Zohr University, PO Box: 1136/S, 80000, Agadir, Morocco

JAMES NICHOLAS FURZE

Laboratory of Biotechnology and Valorisation of Natural Resources, Department of Biology, Faculty of Sciences of Agadir, Ibn Zohr University,  BP 8106, 80000 Agadir, Morocco

NIAMA HEIMEUR

Laboratory of Plant Biotechnology, Department of Biology, Faculty of Sciences of Agadir, Ibn Zohr University, BP 8106, 80000 Agadir, Morocco

BTISSAM SENHAJI

 Biotechnology and Environmental Engineering Team, Laboratory of Mechanic Process Energy and Environment, National School of Applied Sciences, Ibn Zohr University, PO Box: 1136/S, 80000, Agadir, Morocco

BOUCHRA CHEBLI

 Biotechnology and Environmental Engineering Team, Laboratory of Mechanic Process Energy and Environment, National School of Applied Sciences, Ibn Zohr University, PO Box: 1136/S, 80000, Agadir, Morocco

MILOUD EL HADEK

Laboratory of Chemical Engineering, Department of chemistry, Faculty of Sciences of Agadir, Ibn Zohr University, 80000 Agadir, Morocco

THIERRY MATEILLE

 IRD, UMR, CBGP, 755 Avenue du Campus Agropolis, CS30016, 34988 Montferrier-sur-Lez Cedex, France

LALLA AMINA IDRISSI HASSANI

 Laboratory of Plant Biotechnology, Department of Biology, Faculty of Sciences of Agadir, Ibn Zohr University, BP 8106, 80000 Agadir, Morocco

ZAHRA FERJI

Department of Plant Protection, Nematology. Agronomic and Veterinary Institute Hassan II, Agadir, Morocco

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Published

2019-03-05

Issue

Vol. 6 No. 1 (2019): Journal of AgriSearch

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Articles

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